Twitch Prime has existed for about 2 years now, and one of the integral benefits of the subscription-based service has been an ad-free experience. Today, however, an official Twitch communication has stated that the ad-free portion of Prime will be shuttered within the next 2 months. Users who obtain a Twitch Prime subscription after September 14, 2018 will receive ads, while existing monthly subscriptions will only be ad-free until October 15. Annual Twitch Prime subscriptions will be ad-free until they renew, at which point commercials will once again be part of streams.

Source: Twitch